. 2016 Aug 26;16(1):450. doi: 10.1186/s12879-016-1694-9

Table 2.

Assay results of the SD BIOLINE HIV/syphilis Duo test for dual detection of HIV and syphilis, Kathmandu, Nepal, 2014

RDT	Infection	RDT Result	Reference method^a
RDT	Infection	RDT Result	Positive	Negative	Total
Using SD Bioline HIV/Syphilis duo test kit	Syphilis	Positive	42	13	55
Using SD Bioline HIV/Syphilis duo test kit	Syphilis	Negative	2	9943	9945
Total					10,000
	HIV	Positive	19	0	19
	HIV	Negative	0	9981	9981
Total					10,000

RDT rapid diagnostic test

^aTested by using national HIV testing algorithm [7] with a combination of Determine HIV-1/2/O assay (Abbott Laboratories, Abbott Park, IL), Uni-Gold Recombinant HIV-1/2 (Trinity Biotech, Bray, Ireland) HIV 1/2 Stat-Pak Ultra Fast (Chembio Diagnostic Systems, Medford, NY). Testing for syphilis was done, first by RPR test by using IMMUTREP RPR test kit (Omega Diagnostics Scotland UK) and all test positive samples were re-confirmed by TPHA method. (Thirteen (0.13 %) cases in syphilis testing were found false positive while two were false negative and in case of HIV all of 19 cases tested positive using SD Bioline HIV/Syphilis duo test kit also tested positive by the reference method.)