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Limit of detection (LOD) of the Bio-Plex Pro™ rat cytokine assays (Bio-Rad Laboratories, Hercules, CA, USA)
| Targets | Assay sensitivity, pg/mL, LOD (limit of detection) |
|---|---|
| IL-1α | 1 |
| IL-1β | 2 |
| IL-2 | 3 |
| IL-4 | 1 |
| IL-5 | 6 |
| IL-6 | 10 |
| IL-12 | 0.7 |
| TNF-α | 3 |
| GM-CSF | 0.6 |
| MIP-1α | 12 |
| MCP-1 | 4 |
