Fig. 3.

miR-17-92 cluster modulates JAK2-STAT3 signaling pathway. a Seventy-two hours after infecting them with lentivirus overexpressing miR-17-92 cluster (Lenti-miR-17-92) or control lentivirus (Lenti-CON), NSCs were cultured in the different differential media (DMEM/F12/1 % N2 plus media supplement medium alone (CON) or with 10 ng/ml LIF, CNTF or CM) for 4 days. Expression levels of p-JAK2, JAK2, p-STAT3, STAT3, and GFAP were analyzed by western blot. GAPDH was used as an internal control. b Quantification of the protein expression levels from the experiment shown in a. Values are means ± SEM. Asterisks indicate a statistically significant difference compared with the Lenti-CON group (*P < 0.05, **P < 0.01, unpaired two-tailed t test, n = 3 independent experiments). Pound signs indicate a statistically significant difference compared with the untreated control in the same group (^# P < 0.05, ^## P < 0.01, ^### P < 0.001, one-way ANOVA followed by Tukey’s test, n = 3 independent experiments)