Fig. 4.

Augmented activation of γδ T cells by caspase-inhibited DC involves a soluble factor. a γδ T cells were cultured with supernatants (SN) previously obtained from 20-hour cultures of DC plus B. burgdorferi (Bb) with either DMSO or zVAD (50 μM). After an additional 20 h, the γδ T cell supernatants were assayed for IL-17 and IFN-γ. b Transwell cultures were established in which γδ T cells were cultured in the lower chamber and DC cultured with B. burgdorferi in the upper chamber in the absence or presence of zVAD. After 20 h, supernatants from triplicate cultures (mean ± SD) were evaluated for IL-17 and IFN-γ (* p < 0.05 by one-way ANOVA followed by Tukey's post hoc test). Data were consistent in 3 experiments; 2 mice were used in each experiment.