Fig. 5.

IL-4 induces c-FLIP and caspase-8 activity in DC. DC were cultured in GM-CSF in the absence or presence of IL-4. After 7 days, lysates were made containing biotin-VAD (bVAD) to bind active caspases. Active caspases were then selectively precipitated using avidin-sepharose (bVAD ppt) and compared to whole-cell lysates (WCL) by Western blot for c-FLIP, caspase-8, caspase-9 and caspase-3. The results were consistent in 3 experiments; 3 mice were used in each experiment. Densitometry represents a compilation of the 3 experiments.