Fig. 6.

zVAD inhibits caspase-mediated RIPK1 cleavage and induces DC cell death with GM-CSF but not with IL-4. DC cultured for 7 days in GM-CSF or GM-CSF + IL-4 were analyzed in the absence (DMSO) or presence of zVAD for cleavage of RIPK1 by Western blot of whole-cell lysates (a) or cell death (b). c Cell death of GM-CSF-cultivated DC was assessed following culture for 20 h in the absence (DMSO) or presence of the RIPK1-inhibitor, necrostatin (necro; 50 μM), in the absence or presence of zVAD (50 μM). d Cultures containing γδ T cells, DC and B. burgdorferi (Bb) were treated with either control DMSO, zVAD alone or zVAD + necrostatin. Supernatants were assayed after 20 h for the indicated cytokines (* p < 0.05 by one-way ANOVA followed by Tukey's post hoc test). Data were similar in 3 experiments; 4 mice were used in each experiment. Densitometry represents a compilation of the 3 experiments.