Fig. 7.

IL-4 inhibits the ability of DC to activate γδ T cells. DC grown with either GM-CSF or GM-CSF + IL-4 were mixed at a 1:1 ratio with γδ T cells in the absence or presence of B. burgdorferi (Bb) and zVAD. After 20 h, supernatants from triplicate cultures (mean ± SD) were analyzed by Bio-Plex for IL-17 (a), IFN-γ (b) and surface CD25 (c) on the γδ T cells measured by flow cytometry (* p < 0.05 by one-way ANOVA followed by Tukey's post hoc test). Findings were consistent in 3 experiments; 3-4 mice were used in each experiment. AU = Arbitrary units; MFI = mean fluorescence intesity.