Figure 4.

Specific Deletion of Otulin in Myeloid Cells, but Not T or B Cells, Causes Systemic Inflammation

(A, C, and E) Spleens and spleen weights, thymuses, and inguinal lymph nodes from (A) 2- to 3-months-old CD4Cre-Otulin^flox mice (n = 6), (C) 3- to 4-months-old MB1Cre-Otulin^flox mice (n = 4), (E) 3- to 9-months-old LysMCre-Otulin^flox mice (n = 8).

(B, D, and F) Blood cell counts from (B) 2- to 3-months-old CD4Cre-Otulin^flox mice (n = 6); (D) 3- to 4-months-old MB1Cre-Otulin^flox mice (n = 7); and (F) 3- to 9-months-old LysMCre-Otulin^flox mice (n = 11).

(G and H) Luminex multiplex analysis of serum cytokine and chemokine concentrations from terminal bleeds of 2- to 3-months-old CD4Cre-Otulin^flox mice, 3- to 4-months-old MB1Cre-Otulin^flox mice, and 4- to 9-months-old LysMCre-Otulin^flox mice presented as (G) a heatmap of relative changes of analytes between OTULIN-deficient mice and their respective +/flox controls and (H) serum concentrations of selected cytokines and chemokines increased in LysMCre-Otulin^LacZ/flox mice.

(I) ELISA measurements of total IgG concentrations in serum from CD4Cre-Otulin^flox (n = 6), MB1Cre-Otulin^flox (n = 7), and LysMCre-Otulin^flox (n = 14) mice.

Data are presented as mean ± SEM, and n represents number of mice. See also Figure S4 and Tables S5–S7.