Figure S3.

Emergency Granulopoiesis and Neutrophilia Is Controlled by G-CSF in OTULIN-Deficient Mice, Related to Figure 3

(A) Flow cytometry analysis of lineage negative (Lin^-) c-Kit⁺Sca1⁺ LSK cells in bone marrow of tamoxifen-treated CreERT2-Otulin^flox chimeric mice shows increased numbers of LSK cells in bone marrow consistent with emergency granulopoiesis. (B-C) Flow cytometry analysis of mature and immature neutrophils in (B) bone marrow and (C) blood of tamoxifen-treated CreERT2-Otulin^flox chimeric mice shows increased numbers of these cells in both tissues, consistent with emergency granulopoiesis. (D-F) Quantification of flow cytometry analysis as in (A-C) of LSK cells (D) and mature and immature neutrophils in (E) bone marrow and (F) blood of tamoxifen-treated CreERT2-Otulin^flox chimeric mice injected with anti-G-CSF neutralizing antibodies, anti-IL-6 neutralizing antibodies, or isotype control as indicated. (G-I) Serum concentrations of TNF, G-CSF, IL-6, KC, and MCP-1 from tamoxifen-treated CreERT2-Otulin^flox chimeric mice injected with (G) anti-TNF neutralizing antibodies, (H) anti-G-CSF neutralizing antibodies, (I) anti-IL-6 neutralizing antibodies, or isotype control as indicated measured by Luminex multiplex analysis. These data are represented as a heat map in Figure 3J. Data are presented as mean ± SEM, and n represents number of mice.