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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: J Surg Res. 2016 Jun 8;204(2):467–474. doi: 10.1016/j.jss.2016.06.003

Figure 2. The amount of Matrigel a mammary fat pad can hold without spillage.

Figure 2

Trypan blue stained Matrigel was injected into mammary fat pads in 10 μl increments and spillage was detected visually. (A) Representative images of Trypan Blue stained Matrigel inoculation. Spillage out of the mammary fat pads is observed in the 20 μl injection in #2 (left) and 30μl injection in #4 (right) mammary fat pads. (B) The amount of Matrigel that began to spill out of the pads when inoculated to either #2 or #4 mammary fat pads.