6C8 specifically binds B-cells and B-cell lymphoma cells. (A-D) Representative examples of two-dimensional flow cytometry dot plot analysis of canine peripheral blood lymphocytes (PBL), primary canine B-cell lymphoma, T-cell lymphoma, and CML, using 6C8 and antibodies for lymphocyte markers (CD3 and CD22) are shown. (E) One-dimensional histogram of 6C8 binding to CLBL1 cells where the dashed line, the dotted line, and the solid line represent no antibody, isotype control antibody, and 6C8 staining, respectively. Phenotyping results of all lymphoma samples tested are summarized in Supplementary Table I.