(A) Following siRNA-mediated KD of sortilin, three clones of McA cells were selected representing high (H, McA60), medium (M, McA62) and low (L, McA53) sortilin expression evaluated by immunoblotting compared with wild type (WT) McA cells. The effect of increasing sortilin KD on VLDL-B100 secretion by McA cells incubated in cDMEM (B) or incubated in 1% BSA/DMEM (C). Results in (B) and (C) are averages of triplicate plates for each condition (n = 2 studies). * Indicates means are significantly different from the SCR McA cell line.