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. Author manuscript; available in PMC: 2017 Sep 16.
Published in final edited form as: Biochem Biophys Res Commun. 2016 Aug 2;478(2):546–552. doi: 10.1016/j.bbrc.2016.07.096

Fig. 4. Effect of cpd984 on VLDL-B100 secretion and on insulin sensitivity in McA cells.

Fig. 4

(A) McA cell lines with variable sortilin KD were incubated with vehicle (black bars) or with 10 μM cpd984 (gray bars) for 18 h (3-100 mm plates per condition). Viability of McA cells was not compromised by incubations with cpd984 as there was no significant release of LDH into the medium compared with control incubations. VLDL was isolated from media of each plate and VLDL-B100 was quantified by immuno slot blotting. Results presented are averages of the 3 plates ± S.D. (B) McA cells were incubated for 18 h in 1% BSA/DMEM ± 10 μM cpd984. McA cells were then stimulated with a time course of 250 nM insulin (0, 5, 10 and 15 min). IRβ and AKT, pY-IRβ, and p-AKT (S473) were evaluated by immunoblotting using protein and phosphospecific antibodies.