Table 5.
Growth rates of Synechocystis wild type, ΔggpS and ΔglpK divided by the growth rate of Δslr1670.
| μ/μ [%] |
|||||||
|---|---|---|---|---|---|---|---|
| Interval [days] | qGG [mmol ⋅gDW-1 h-1] | WTΔslr1670 |
ΔggpSΔslr1670 |
ΔglpKΔslr1670 |
|||
| sim.i | exp.ii | sim.i | exp.ii | sim.i | exp.ii | ||
| 0–2 | 0.008 | 103.9 | 100.9 ± 2.4 | 103.9 | 105.7 ± 2.3 | 103.9 | 102.8 ± 3.5 |
| 2–4 | 0.015 | 107.4 | 109.4 ± 11.0 | 107.4 | 106.1 ± 12.2 | 107.4 | 104.6 ± 10.3 |
| 4–6 | 0.014 | 106.9 | 122.8 ± 5.6 | 106.9 | 123.3 ± 5.4 | 106.9 | 128.7 ± 11.2 |
| 6–8 | 0.009 | 104.6 | -73.7 ± 36.8 | 104.6 | -42.8 ± 24.6 | 104.6 | -106.9 ± 21.7 |
| 8–10 | 0.003 | 101.5 | 135.7 ± 41.8 | 101.5 | 93.0 ± 31.6 | 101.5 | 120.9 ± 59.8 |
qGG values were calculated using glycerol production data of the ΔggpS mutant (Figure 5).
i sim.: simulated; at physiological uptake fluxes of GG, the model does not predict a difference for the mode of cleavage (phosphorolytic versus hydrolytic, Supplementary Figure S2A). Similarly, utilization of the glycerol part does not lead to increased growth rate (Supplementary Figure S2B). ii exp.: experimental; error margins represent standard deviations of three replicates.