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. 2016 Aug 29;7:1349. doi: 10.3389/fmicb.2016.01349

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Copyright © 2016 Qian, Zhong, Hou, Qu and Zhong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Deletion of pyr4 gene in T. reesei SN1. (A) Schematic representation of the pyr4 locus in the Δpyr4 and parent strains. Relative positions of the XbaI/HindIII enzyme restriction sites are noted. Probe used for Southern analysis is shown as red box. (B) Phenotypic assay on MM supplemented with uracil (left) and MM without uracil (right) at 30°C for 2 days. (C) Southern blot of the genome digested with XbaI/HindIII. A fragment of 4.7 kb is present in the parent strain, the 6.0 kb band is shown in Δpyr4 strains.