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. 2016 Aug 29;7:1349. doi: 10.3389/fmicb.2016.01349

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Copyright © 2016 Qian, Zhong, Hou, Qu and Zhong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overexpression of bgl1 gene in the Δpyr4 mutant. (A) An expression cassette containing the bgl1 gene is controlled by the T. reesei modified cbh1 promoter. Relative positions of the EcoRI enzyme restriction sites are noted. Probe used for Southern analysis is shown as red box. (B) PCR assay of the BGL overexpression transformants. Both the transformants SPB1 and SPB2 show a 1.0 kb DNA fragments panning using the primers Y1 and Y2, M: DNA marker. (C) Southern blot of the genome digested with EcoR1. The 1.8 kb (red line) wild-type band is present in all the strains. The additional bands indicate the bgl1-overexpression cassette was successfully integrated into the chromosome.