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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Mol Microbiol. 2015 Sep 29;98(5):910–929. doi: 10.1111/mmi.13168

Figure 7. Induction of TRB3 mRNA is dependent on CBP1 and precedes macrophage lysis.

Figure 7

(A) BMDMs were infected with G217B yeast cells at an MOI of 5. At various time points post-infection, total RNA was isolated from BMDMs and TRB3 expression was monitored by quantitative RT-PCR. Fold induction was calculated relative to the uninfected 0-hour sample. (B) To monitor macrophage lysis, supernatants from the same infected macrophages assayed in (A) were subjected to LDH release analysis. (C) BMDMs were either mock-infected (uninfected) or infected with wild-type G217B ura5Δ+URA (wild-type) or cbp1+URA mutant cells (cbp1) at an MOI of 10. Lysis of the macrophage monolayer infected with wild-type cells was complete by 48 hpi. At the indicated times post-infection, total RNA was isolated from BMDMs and TRB3 expression was monitored by quantitative RT-PCR. Fold induction was calculated relative to the mock-infected 24-hour sample. (D) BMDMs were either mock-infected (uninfected) or infected with wild-type G217B ura5Δ+URA (wild-type), cbp1+URA mutant cells (cbp1), or the complemented strain (cbp1+CBP1) at an MOI of 2. At the indicated times post-infection, total RNA was isolated from BMDMs and TRB3 expression was monitored by quantitative RT-PCR. Fold induction was calculated relative to the mock-infected 0-hour sample.