Figure 3. VR1 agonists increased OVA-specific CTL activity in mice injected with VV-OVA. Mice were orally administered CP or RTX for 3 days, infected with VV-OVA, and then treated with the same doses of CP or RTX for consecutive 5 days, as described in Fig. 1. On the final day of VR1 agonist administration, and in vivo CTL assays were performed using CFSE-labeled syngeneic target cells. (A) Representative histograms of lymph node and spleen cells, and the percentage of specific killing of OVA[257-264] peptide-pulsed target cells in the lymph nodes and spleens are shown (n=5 for each group). Each data point represents the mean±SD of values obtained from 2 individual experiments. ^*p<0.05; ^**p<0.01.