Figure 6. VR1 agonists increases class I, but not class II, MHC-restricted presentation of exogenous antigen in BM-DCs. (A) BM-DCs generated from BM cells of C57BL/6 mice were treated with the indicated concentrations of CP or RTX for 4 h, and then allowed to phagocytose OVA-microspheres for 2 h. After fixing, the amounts of H-2Kb-OVA peptide complexes were assessed using CD8 OVA 1.3 cells. (B) BM-DCs generated from BM cells of BALB/c mice were treated with the indicated concentrations of CP or RTX for 4 h, and then allowed to phagocytose OVA-microspheres for 2 h. After fixing, the amounts of I-Ad-OVA peptide complexes were assessed using DOBW cells. (C, D) Effects of CP and RTX on phagocytic activity. BM-DCs generated from BM cells of C57BL/6 mice were incubated with FITC-labeled OVA-microspheres, washed, harvested, and analyzed by flow cytometry. A representative set of histograms from 3 individual experiments is shown. Shaded histograms, phagocytic activity of DCs in the absence of CP or RTX; thick line histograms, phagocytic activity of DCs in the presence of CP or RTX.
