Figure 5.

Hyperinsulinemia increases phosphorylated eNOS protein expression in skeletal muscle of lean healthy controls (LHCs) (n = 10) but not in T2DM (n = 8). Western blot analysis of total eNOS (A) and p‐eNOS (serine 1177) protein expression (B) in skeletal muscle homogenate. Signal relative intensity was normalized to β‐actin and results represent the means ± SE for each group. (C) Ratio between the normalized expression of p‐eNOS and eNOS proteins in T2DM subjects and LHCs at 2 h of hyperinsulinemia. Correlation between clamp‐derived glucose disposal rate in study subjects and basal expression of eNOS protein (D) and Δ p‐eNOS (E). *P < 0.05 for insulin versus basal and † (P < 0.05) for LHCs versus T2DM.