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. 2016 Aug 29;7:322. doi: 10.3389/fimmu.2016.00322

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Copyright © 2016 Lund, Boysen, Åkesson, Lewandowska-Sabat and Storset.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Percentages of monocytes in LNs and PBMC. (A) Percentages of CD14⁺⁺ CD16⁺ monocytes of total live cells in LNs of non-injected animals, and in draining and contralateral LNs of injected animals at 24, 48, and 96 h post-injection. Symbols represent individual animals and the median value within each group is depicted as a line. Statistical significant differences between injected and non-injected groups using the Wilcoxon rank-sum test are indicated as *P < 0.05. Statistical significant differences between groups consisting of the same individuals (identical symbols) using the paired t-test are indicated as ^#P < 0.05. (B) Percentage of CD14⁺ monocytes of live cells in PBMC at pre-injection, and at 24, 48, 72, and 96 h post-injection. Symbols and statistics as in (A) (^#P < 0.05). (C) Percentage of CD14⁺⁺ CD16⁺ monocytes of live cells in the draining high dose (subiliac) LN and the contralateral LN, and in the draining low-dose (superficial cervical) LN. Symbols and statistics as in A (^#P < 0.05). (D) Percentage of CD14⁺ monocytes of total live cells in PBMC at pre-injection and after 24 h. Symbols and statistics as in A.