Figure 6.

Distribution of monocyte and DC markers in skin and LNs. Immunofluorescent labeling of subcutaneous tissues and LNs with antibodies against CD14 (green), CD205 (red) and CD11c (blue), at 24 h post-injection. (A) Large numbers of CD14⁺ cells were observed in the subcutis on the injected side. Note the separation of collagen bundles (appears as gray auto fluorescence, arrowheads) due to inflammatory infiltrates. A limited number of CD205⁺ cells were present. (B) A few CD14⁺ cells (arrow) were observed on the contralateral side. (C) CD14⁺ cells infiltrated the interfollicular areas of the cortex of the draining LN. A moderate amount of CD205⁺ CD11c⁺ DCs were observed (arrow heads in insert). (D) CD14⁺ cells were sparsely present in the contralateral LN. CD205⁺ follicles were surrounded by CD11c single labeled and CD11c⁺ CD205⁺ double labeled cells. Follicle (f), interfollicular area (i), capsule (c), sinus area (s). Scale bars: 100 μm.