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. 2016 Aug 29;7:322. doi: 10.3389/fimmu.2016.00322

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Copyright © 2016 Lund, Boysen, Åkesson, Lewandowska-Sabat and Storset.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gene expression of monocytes from the draining LN. Gene expression levels (normalized to the reference gene PPIA) of CD14⁺ cells isolated from blood (baseline non-injected) and from the draining LN at 24 h post-injection, as analyzed by RT-qPCR. Symbols represent individual animals and the median value within each group is depicted as a line. Statistical significant differences between the two groups using the unpaired t-test (for normally distributed data) are indicated as ^#P ≤ 0.05, and the Wilcoxon rank-sum test (for non-normally distributed data) are indicated as *P ≤ 0.05. (A) Gene expression levels of CD14, CD16α, IL-1β, and TGFβ. (B) Gene expression levels of IL-12β, TNFα, IL-6, and IL-10. Note the difference in y-axis range from (A). (C) Gene expression levels of chemokine receptors CCR2 and CX₃CR1. Note the difference in y-axis range from (A,B).