Table 1.
List of primers used for reverse transcription-quantitative PCR (RT-qPCR).
| Gene symbol, accession no. | Primers (5′ → 3′) | Amp (bp) | Reference | |
|---|---|---|---|---|
| CD14, NM_174008.1 | CGATTTCCGTTGTGTCTGC | 150 | (16) | |
| TACTGCTTCGGGTTGGTGT | ||||
| CD16α, NM_001077402.1 | Low-affinity FcγIIIR | TGTCTCGTCATTCTTTCTACCTTG | 138 | (16) |
| ACTTTGCCATCCCTCCATTC | ||||
| CX3CR1, NM_001102558.2 | CX3C-chemokine receptor 1 | TCACCAGAGAGAAAGAGAACGA | 108 | (16) |
| GGAGCAGGAAGCCAAGAAA | ||||
| CCR2, NM_001194959 | CC-chemokine receptor 2 | GATGAAGAACCCACCACCAG | 118 | (16) |
| CAAAGATGAAGACCAGCGAGTAG | ||||
| TGFβ1, NM_001166068.1 | Transforming growth factor beta 1 | CAATTCCTGGCGCTACCTCA | 121 | Primer 3 |
| GCCCTCTATTTCCTCTCTGCG | ||||
| IL-1β, NM_174093.1 | Interleukin-1 beta | AAAAATCCCTGGTGCTGGCT | 89 | Primer 3 |
| CATGCAGAACACCACTTCTCG | ||||
| IL-6, NM_173923.2 | Interleukin-6 | CCTGAAGCAAAAGATCGCAGA | 97 | Primer 3 |
| TGCGTTCTTTACCCACTCGT | ||||
| IL-10, NM_174088.1 | Interleukin-10 | TATCCACTTGCCAACCAGCC | 152 | Primer 3 |
| GGCAACCCAGGTAACCCTTA | ||||
| IL-12β, NM_174356.1 | Interleukin-12 subunit beta | GAGGTCGTGGTAGAAGCTGT | 87 | Primer 3 |
| TGGGTCTGGTTTGATGATGTCC | ||||
| TNFα, NM_173966.3 | Tumor necrosis factor alpha | TCTTCTCAAGCCTCAAGTAACAAG | 103 | (27) |
| CCATGAGGGCATTGGCATAC |
Amp, amplicon.