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. 2016 Aug 1;30(15):1761–1775. doi: 10.1101/gad.281030.116

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© 2016 Fu et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 2. — Impaired dPER rhythms in OP1 flies. (A) Western blot results showing the dPER molecular rhythm in LD (top) and DD (bottom) for wild-type and OP1 flies. The filled and open arrowheads indicate the hyperphosphorylated and hypophosphorylated dPER proteins, respectively. Membrane staining was used as a loading control. (B) Densitometric analyses of the results from three independent experiments. The levels of dPER were normalized to the loading control. Error bars indicate ±SD. (C) Immunohistochemistry assay of dPER expression in pigment dispersing factor (PDF)-positive (PDF⁺) circadian neurons in fly brains. Adult flies were entrained to LD cycle, and brains were dissected for immunohistochemistry analysis at the indicated time points.