Figure 1.

Class IV da neurons regenerate branch number, but not territory coverage, after all dendrites are severed at 48 h after egg laying (AEL). (A) Schema of experiments in this study. After synchronized egg laying, neurons were either balded or uninjured, usually at 48 or 72 h AEL. Neurons were mounted and imaged ∼24 h after injury or mock injury to confirm that all dendrites had been severed and again 72 h after injury or mock injury to assess regeneration. (B) Representative images of ddaC neurons when adjacent anterior and posterior neurons have been ablated 24 h (left) and 72 h (right) after either balding (bottom) or mock injury (top). (C,D) By 72 h after balding (blue), neurons regenerate the number of branches and almost regenerate the arbor length of an uninjured neuron. (E) Neurons recover only about half of the body wall territory of an uninjured neuron. (F) Sholl analysis of dendrite complexity 72 h after injury for balded (solid) and control (dashed) neurons. (**) P < 0.0001; (ns) P > 0.05, by one-way ANOVA with Tukey's multiple comparisons test. Bar, 50 μm. n = 16 balded neurons and 15 mock neurons.