Figure 6.

Class IV dendrite balding and regeneration do not permanently alter expression of the class-specific transcription factor cut, receptor sorting and trafficking, glial wrapping, or epidermal cells. (A) Immunostaining for the transcription factor cut did not show significant differences in expression level between neurons 6 h after balding versus control cells. (B,C) Immunostaining for ppk1 (B) and ppk26 (C) show sorting and trafficking of the mechanoreceptors into the somatodendritic compartment and exclusion from the axon in both uninjured control neurons and neurons 72 h after balding and regeneration. (D) Glial cells, marked by the repo promoter driving expression of CD4-tdTomato, wrap the axon and cell body at 24 h after balding and also the proximal dendrites of both control neurons and regenerated neurons at 72 h after balding. (E) Adjacent epidermal cells whose borders are marked by β-catenin arm^GFP are sometimes uninjured by balding (middle row) and sometimes ablated by the balding injury (bottom row), but neurons are capable of regenerating dendrites in either case. Bars: A,D, 10 µm; B,C,E, 50 µm.