Table 1:
Figures | Comparisons | Label | Data structure | Type of test | Power |
---|---|---|---|---|---|
1F | PBS vs HGF (5 min) | a | Normal distribution after transformation | Two-way ANOVA with Bonferroni's multiple comparisons test | 0.26 |
PBS vs HGF (10 min) | b | 1.00 | |||
PBS vs HGF (30 min) | c | 0.93 | |||
2C | PBS vs HGF | d | Non-normal distribution | Mann–Whitney test | 0.99 |
3C | PBS vs HGF | e | Normal distribution | Unpaired t test with Welch's correction | 1.00 |
4D | β-catenin vs β-cateninY142F PBS treatment) | f | Normal distribution after transformation | Two-way ANOVA Bonferroni’s multiple comparisons test | 0.17 |
β-catenin vs β-cateninY142F HGF treatment) | g | 1.00 | |||
PBS vs HGF (β-catenin transfection) | h | 1.00 | |||
PBS vs HGF (β-cateninY142F transfection) | i | 0.01 | |||
5E | PBS vs HGF (Syn-GFP/bassoon) | j | Non-normal distribution | Mann–Whitney test | 0.99 |
PBS vs HGF (Syn-GFP/PSD-95) | k | Non-normal distribution | Mann–Whitney test | 0.99 | |
PBS vs HGF (Syn-GFP) | l | Non-normal distribution | Mann–Whitney test | 0.94 | |
5F | PBS vs HGF (Syn-GFP/bassoon) | m | Non-normal distribution | Mann–Whitney test | 0.94 |
PBS vs HGF (Syn-GFP/PSD-95) | n | Non-normal distribution | Mann–Whitney test | 0.78 | |
PBS vs HGF (Syn-GFP) | o | Non-normal distribution | Mann–Whitney test | 0.82 | |
5G | PBS vs HGF (Syn-GFP/bassoon) | p | Non-normal distribution | Mann–Whitney test | 0.90 |
PBS vs HGF (Syn-GFP/PSD-95) | q | Non-normal distribution | Mann–Whitney test | 0.75 | |
PBS vs HGF (Syn-GFP) | r | Normal distribution | Unpaired t test with Welch's correction | 0.92 | |
5H | PBS vs HGF (Syn-GFP/bassoon) | s | Non-normal distribution | Mann–Whitney test | 0.91 |
PBS vs HGF (Syn-GFP/PSD-95) | t | Non-normal distribution | Mann–Whitney test | 0.90 | |
PBS vs HGF (Syn-GFP) | u | Non-normal distribution | Mann–Whitney test | 0.88 | |
6C | β-catenin vs β-cateninY142F PBS treatment, Syn-GFP/bassoon) | v | Normal distribution after transformation | Two-way ANOVA Bonferroni's multiple comparisons test | 0.00 |
PBS vs HGF (β-catenin transfection, Syn-GFP/bassoon) | w | 0.98 | |||
PBS vs HGF (β-cateninY142F transfection, Syn-GFP/bassoon) | x | 0.69 | |||
6D | β-catenin vs β-catenin Y142F PBS treatment, Syn-GFP/PSD-95) | y | Normal distribution after transformation | Two-way ANOVA Bonferroni's multiple comparisons test | 0.00 |
PBS vs HGF (β-catenin transfection, Syn-GFP/PSD-95) | z | 1.00 | |||
PBS vs HGF (β-cateninY142F transfection, Syn-GFP/PSD-95) | aa | 0.00 |
To meet the assumption of normality, the data from Figures 1F, 4D, and 6C were transformed with the square root, and the data from Figure 6D were transformed with the natural logarithm. The normality of the data was tested using the D’Agostino and Pearson omnibus normality test.