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. 2016 Aug 29;3(4):ENEURO.0148-16.2016. doi: 10.1523/ENEURO.0148-16.2016

Figure 1.

Figure 1.

Arf6 is required for cortical neuronal migration. A, Representative micrographs of E17.5 cerebral cortices electroporated with the pNeuroD plasmids carrying Mock, Arf6Q67L, or Arf6T44N in combination with pCAGGS-EGFP at E14.5 (n = 5 embryos). B, Immunoblot analysis of cultured cortical neurons transfected with Arf6 shRNA (Arf6 KD) or its scramble shRNA (Control) for 3 d. The numbers indicate the percentage of endogenous Arf6 expression level relative to α-tubulin. C, Representative micrographs of E17.5 cerebral cortices electroporated with the pmU6pro plasmids carrying scramble sequence (Control) or Arf6 shRNA (Arf6 KD) in combination with pCAGGS-EGFP at E14.5 (n = 4 embryos). D, Representative micrographs of P0 cerebral cortices electroporated with Control, Arf6 KD, or Arf6 KD and Arf6WT plasmids in combination with pCAGGS-EGFP at E14.5 (n = 4 embryos). Bottom graphs in A, C, and D show the quantification of the distribution of EGFP-positive cells in cortical layers. Data were presented as mean ± SEM and statistically analyzed using one-way ANOVA followed by Tukey–Kramer’s tests in A and D (*P < 0.05, **P < 0.01), and by unpaired t test in C (*P < 0.05, **P < 0.005, ***P < 0.0005). n in the graph indicates the number of embryos examined. Scale bars: A, C, D, 200 μm.