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. 2016 Aug 29;3(4):ENEURO.0148-16.2016. doi: 10.1523/ENEURO.0148-16.2016

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Copyright © 2016 Hara et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 2. — Effects of the knockdown of Arf6 on cell proliferation and neuronal differentiation. A, Representative micrographs showing the distribution of BrdU-positive cells in the VZ. Embryos were electroporated with the indicated shRNAs and EGFP at E14.5 and killed at E15.5 1 h after the intraperitoneal administration of BrdU to pregnant mice. Arrowheads show BrdU-incorporated mCherry-positive cells in the VZ. Note no apparent differences in the cell number or position of BrdU-positive transfected cells between Control and Arf6 knockdown (Arf6 KD). B, Representative micrographs showing the expression of PSA-NCAM in control and Arf6-KD neurons in the IZ at E17.5. C, Representative micrographs showing that the expression of Cux1 in control and Arf6-KD neurons in the superficial cortical layers at P0. Scale bars: A, 25 μm; B, 50 μm; C, 100 μm.