Figure 6.

Arf6 regulates neuronal migration through the interaction with class II FIPs. A, Representative micrographs showing rescue experiments of impaired neuronal migration caused by the knockdown of Arf6 with separation-of-function Arf6 mutants. Brains were electroporated with the indicated pCAGGS plasmids carrying Mock, Arf6^iSW, Arf6^N48I, Arf6^W168L/L169V in combination with Arf6 shRNA and pCAGGS-EGFP at E14.5, and fixed at P0. Bottom graphs show the quantification of the distribution of EGFP-positive cells in cortical layers. B, Comparison of the percentage of EGFP-positive cells transfected with the indicated plasmids in the superficial layers II–IV at P0. Data were presented as mean ± SEM and statistically analyzed using one-way ANOVA followed by post hoc Tukey-Kramer’s test (*P < 0.05). n in the graph indicates the number of embryos examined. Scale bar, 200 μm.