Figure 9.

FIP3 regulates the trafficking of N-cadherin in migrating neurons through the interaction with Arf6 and Rab11. A, Representative micrographs showing the effect of the expression of FIP3 shRNA with or without the indicated FIP3 mutants on the subcellular localization of N-cadherin in migrating neurons in the IZ at E17.5. Arrowheads indicate the localization of N-cadherin in transfected neurons. B, Quantification of the relative immunofluorescence intensity for cytoplasmic N-cadherin. The relative intensity was calculated by normalizing the immunofluorescence intensity for cytoplasmic N-cadherin in transfected neurons to that in the surrounding untransfected neurons. Note the cytoplasmic accumulation of N-cadherin in neurons transfected with FIP3 KD#2, which was rescued by the coexpression of FIP3^WT, but not FIP3^ΔABD or FIP3^ΔRBD (Control, n = 62 cells; FIP3 KD#2, n = 136 cells; FIP3 KD#2 and FIP3^WT, n = 96 cells; FIP3 KD#2 and FIP3^ΔABD, n = 114 cells; FIP3 KD#2 and FIP3^ΔRBD, n = 117 cells). Data collected from three embryos in each condition were presented as mean ± SD and statistically analyzed using one-way ANOVA followed by post hoc Tukey–Kramer’s test (*P < 0.05, **P < 0.01). Scale bar, 20 μm.