Table 3.

Antibodies Used for Immunofluorescence Staining

	Protein symbol	Species	Manufacturer	Cat.-No.	Final conc. [μg/mL]
Primary antibody
Alpha fetoprotein	AFP	mouse	Santa Cruz	Sc-8399	2
Albumin	ALB	mouse	Sigma	A6684	67
Cytokeratin 18	CK18	mouse	Santa Cruz	Sc-6259	2
Cytokeratin 19	CK19	rabbit	Santa Cruz	Sc-25724	2
Cytochrome P450 1A2	CYP1A2	mouse	Santa Cruz	Sc-53614	2
Cytochrome P450 2B6	CYP2B6	rabbit	Santa Cruz	Sc-67224	2
Hepatocyte nuclear factor 4 alpha	HNF4A	rabbit	Santa Cruz	Sc-8987	2
Marker of proliferation Ki-67	MKI67	mouse	BD Biosciences	556003	10
Multidrug resistance-associated protein 2	MRP2	rabbit	Sigma	M8316	9
POU domain, class 5, transcription factor 1	OCT3	rabbit	Santa Cruz	Sc-9081	2
Stage-specific embryonic antigen 4	SSEA4	mouse	R&D Systems	MAB1435	10
Tight junction protein 1	TJP1	mouse	LSBio	LS B2228	30
Secondary antibody
Alexa Fluor® 488 anti-mouse		goat	Life Technologies	A-11029	2
Alexa Fluor 594 anti-rabbit		goat	Life Technologies	A-11037	2

Final concentrations are given in μg/mL.