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. 2016 Feb 23;21(12):1717–1725. doi: 10.1038/mp.2016.6

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Copyright © 2016 Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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TSPAN5 and ERICH3 association with serotonin biosynthesis and metabolism. (a) Serotonin biosynthesis and metabolism pathway. (b) mRNA expression of genes encoding serotonin pathway enzymes as measured by qRT-PCR were decreased after TSPAN5 KD (black) and increased after TSPAN5 OE (cross-hatched) in SK-N-BE(2) neuroblastoma cells. (c, d) Western blot analysis indicated decreased serotonin enzyme protein levels after TSPAN5 KD but no significant change after TSPAN5 OE, as quantified in (d). (e, f) Culture media serotonin concentrations after TSPAN5 (e) KD and (f) OE. (g, h) Cell culture media serotonin concentrations after ERICH3 (g) KD and (h) OE. *P<0.05; **P<0.01; TPH1/2, tryptophan hydroxylase 1/2; DDC, dopa decarboxylase; KD, knockdown; MAOA/B, monoamine oxidase A/B; OE, overexpression; SLC6A4, serotonin transporter; EV, empty vector.