Figure 1.
Fluorescence image of a developed HPTLC plate with the area imaged by DESI-MSI outlined in green (a). Identified products of the PZO reaction (Supplementary Scheme SI 1, only 5-isomer is shown), with the numbers indicating the spatial location of each product in the fluorescence and MS images (b). Selected ion images acquired by DESI-MSI of reaction products previously identified (c), and the corresponding spot-like images as they were automatically generated by DetectTLC using the preset Protocol 1 processing pipeline (d)