Figure 1.

miR-210 targets FBXO31 gene in breast cancer.

Notes: (A) In wt human FBXO31 gene, its 3′-UTR DNA sequence included a putative hsa-miR-210 binding site. In a mu FBXO31 3′-UTR, the hsa-miR-210 binding site was point-mutated to another amino acid to avoid binding. In T47D (B) and MCF-7 (C) cells, luciferase vectors of FBXO31(mu), FBXO31(wt), or Renilla control were cotransfected with hsa-miR-210 mimics for 48 hours. A dual-luciferase reporter assay was then carried out (^ΔP>0.05, *P<0.05).

Abbreviations: FBXO31, F-box protein 31; miR-210, microRNA-210; mu, mutant; UTR, untranslated region; wt, wild type.