Table 3. Cysteine residues identified with increased reactivity (≥ 2-fold) in daf-2 relative to daf-16;daf-2 mutants.

The 36 cysteines on 31 proteins that demonstrated increased reactivity in daf-2 (R ≥ 2) are shown. Transcriptomic changes (decreased or increased in daf-2) of the corresponding genes previously identified in microarray analysis of daf-2 and daf-16;daf-2 are shown {McElwee, 2003 #131;McElwee, 2004 #132}. Proteomic changes for these proteins in unenriched proteomic analyses (decreased, increased or no change in daf-2) are also indicated {Depuydt, 2013 #119}. “Not identified” indicates a protein that was not present in the unenriched proteomic datasets.

Gene Symbol	Protein Name	Sequence	Ratio	Function
Y57A10A.23	Thioredoxin domain- containing protein 12#	K.SWC*HACK.A	0.805	Redox-active disulfide
alh-8	Methylmalonate- semialdehyde dehydrogenase	R.C*MALTTAVLVGEAR.A	0.898	Active site nucleophile
alh-12	Aldehyde dehydrogenase#	-AMLANFLNQGQVC*TNATR.V	1.03	Active Site Nucleophile
acaa-2	Acetyl-CoA acyltransferase 2	R.LC*GSGFQAVVNAAQAIK.L	1.15	Acyl-thioester intermediate
ZK669.3	Gamma-interferon- inducible lysosomal thiol reductase#	R.C*SDTSYWMK.W	1.44	Redox-active disulfide
upb-1	Ureidopropionase beta#	R.IGINIC*YGR.H	1.92	Active site nucleophile
gsto-1	Glutathione transferase omega-1	R.FC*PWAER.A	1.96	Active Site Nucleophile
kat-1	Acetyl-CoA acetyltransferase	K.VC*SSGLK.A	2.55	Acyl-thioester intermediate
aco-1	Aconitate hydratase	K.IGFNIAGYGC*MTCIGNSGP-	2.66	Iron-binding
Y71H2AR.1	Thioredoxin domain- containing protein 17#	K.ILTTGESWC*PDCVVAEPVV-	2.76	Active site nucleophile/ Redox-active disulfide
alh-3	Formyltetrahydrofolate dehydrogenase	K.GENC*IAAGR.V	2.79	Active site
acaa-2	Acetyl-CoA acyltransferase 2	K.YGIGSAC*IGGGQGIAILFEK-	2.98	Active site