Fig. 3.

MM6 cells were differentiated with TGFβ and VD3, and A549 cells were starved and stimulated with IL-1β. Supernatants (10,000 g) from total cell lysates were analyzed by Western blots, as described in the Materials and Methods. The 50 μg protein samples were applied to the SDS-PAGE gels; β-actin was control for equal loadings. For each protein, similar results were observed in at least three different experiments.