TABLE 1.

Release of LTC₄ from MM6 cells, MDDCs, and eosinophils

	LTC4 (pmol/106 cells)
	Intracellular	Extracellular	Sum
MM6 incubated LTA4	163 ± 21	90 ± 5	253
MM6 incubated PMA + A23187	9 ± 1	17 ± 1	26
MDDCs incubated LTA4	23 ± 10	33 ± 7	56
Eosinophils incubated LTA4	28 ± 7	44 ± 12	72

Release of LTC₄ from MM6 cells, MDDCs, and eosinophils was analyzed after incubations with LTA₄ (20 μM) for 5 min at 37°C (n = 4–5). MM6 cells were incubated with PMA also (100 nM, 10 min) followed by A2387 (5 μM, 10 min) (n = 6). The incubations were stopped by centrifugation at 4°C (3 min at 1,000 g). Supernatants (extracellular) were rapidly removed and added to 0.5 ml methanol containing 250 pmol PGB₂, while the cell pellet was resuspended in 1 ml methanol:water (1:1), with 250 pmol PGB₂. After extraction, LTC₄ was analyzed by HPLC. Results are mean ± SE.