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. 2016 Sep;57(9):1670–1683. doi: 10.1194/jlr.M067363

Fig. 4.

Fig. 4.

ANGPTL4 does not affect transcription of LPL or secretion of SLURP1. A: Transcript levels for LPL and ANGPTL4 in CHO pgsA-745 cells that had been transfected with empty control vector (C), an expression vector for the Flag-tagged N-terminal domain of hANGPTL4 (NTD), a vector for V5-tagged hLPL (LPL), or N-terminal hANGPTL4 and V5-tagged hLPL (LPL+NTD). B: Western blots of cell lysates of CHO pgsA-745 cells that had been cotransfected with an expression vector for Flag-tagged full-length hANGPTL4 and either a vector for V5-tagged hLPL or a vector for hSLURP1. Western blots were probed with an anti-Flag antibody to detect ANGPTL4 (red), an anti-SLURP1 antiserum (green), an anti-V5 tag antibody to detect LPL (green), and an anti-actin antibody (red) (as a loading control).