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. 2016 Jun 29;4:2800410. doi: 10.1109/JTEHM.2016.2573305

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FIGURE 2. — -LEAF assay principle. a) The -LEAF probe comprises a -lactam core structure (green) including the cleavable lactam ring, flanked by two fluorophores (encircled), which undergo static quenching when the probe is intact. Following cleavage by -lactamase, the fluorophores move apart and show fluorescence. b) Assay profile for -lactamase producing bacteria. c) Assay profile for lactamase non-producing bacteria.

Inline graphic — -LEAF assay principle. a) The -LEAF probe comprises a -lactam core structure (green) including the cleavable lactam ring, flanked by two fluorophores (encircled), which undergo static quenching when the probe is intact. Following cleavage by -lactamase, the fluorophores move apart and show fluorescence. b) Assay profile for -lactamase producing bacteria. c) Assay profile for lactamase non-producing bacteria.