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. Author manuscript; available in PMC: 2017 Feb 4.
Published in final edited form as: Nature. 2016 Aug 4;536(7614):96–99. doi: 10.1038/nature18954

Figure 4. An RNA element inhibits eIF4F recruitment and directs mRNAs to use an eIF3-specialized translation pathway.

Figure 4

a, Schematic of c-Jun 5′ UTR truncation-luciferase reporter mRNAs. SL, stem loop; Luc, luciferase. b, Luciferase activity from in vitro translation of mRNAs containing truncations of the c-Jun 5′ UTR, with or without the internal eIF3-recruitment stem loop sequence. The results are given as the mean and standard deviation (s.d.) of three independent experiments, each performed in triplicate. c, Western blot analysis of initiation factors in 48S translation initiation complexes formed on c-Jun mRNA with a 5′ 153-nucleotide truncation. 293T, total protein from 293T in vitro translation extracts. The result is representative of three independent experiments. For gel source data, see Supplementary Figure 1. d, Model for eIF3d-directed cap-dependent mRNA translation. An eIF4F-inhibitory RNA element ensures that mRNA translation occurs through an eIF3-specialized pathway.