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Representative methylation-specific polymerase chain reaction of promoters in tissues. (a) SFRP2; (b) TFPI2; (c) NDRG4; and (d) BMP3.
NC, negative control; PC, positive control; NN, nonadjacent normal-appearing tissue; AN, adjacent normal-appearing tissue; T, primary colorectal tumor tissue; SFRP2, secreted frizzled-related protein 2; TFPI2, tissue factor pathway inhibitor 2; NDRG4, N-Myc downstream-regulated gene 4; BMP3, bone morphogenic protein 3.
