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Single-channel records of alternate conductance states. Single-channel planar lipid bilayer records of WT PA and various indicated PA mutants in the presence or absence of peptide αl at asymmetric KCl (100 mM [added KCl]cis, 0 mM [added KCl]trans) and at symmetric (A) pH 5.6 or (B) pH 7.6. The O1 and O2 open states and the closed state (C) are indicated. Records are Gaussian-filtered to 50 Hz.
