LF1–50 peptide sequence with stereochemical modifications. (A) (Top) Sequence of LF1–50 (LF residues 1–50) with an amino-terminal six-histidine tag and a carboxyl-terminal tryptophan. The coding sequence of LF is underlined and numbered accordingly. Beneath (Upper Middle to Bottom) are the stereochemical compositions of the αl, αd, and αld peptides, where the l-isomers (white L) and d-isomers (black D) are indicated. The nonchiral glycines are indicated, in gray, by their amino acid symbol “G.” CD spectra of αl (black line), αd (red line), and αld (green line) peptides in the (B) absence and (C) presence of 20% TFE are plotted.