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. 2016 Aug 4;113(34):E5014–E5023. doi: 10.1073/pnas.1607602113

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Fig. 6. — Comparison of divergence levels for antigen processing and presentation genes. To compare the levels of divergence encoded by antigen processing and presentation genes from zebrafish and additional species, we searched for divergent alleles across vertebrates using the BLAST algorithm with expressed sequence tag (EST), transcriptome sequence assembly (TSA), and nonredundant (nr) GenBank databases. The most divergent alleles for each gene were identified and cataloged (accession numbers, divergence levels, and species identifiers are provided in SI Appendix, Table S11). Amino acid divergence (percentage) was calculated between these alleles using BLAST to identify those with the lowest amino acid identity, with divergence (%) = 100 (%) − identity (%). In many cases, these results are likely to underestimate diversity because of limitations, such as undersampling of sequences.