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shRNA-mediated knockdown of caspase-1 (ICE). (A) Western blot of untreated M17 cells (Untr), transfected with control vector carrying a scrambled GFP shRNA (Scr) and an anti–caspase-1 shRNA (RNAi). (B) Levels of caspase-1 mRNA transfected with empty vector (Vec) and anti–caspase-1 shRNAs (RNAi) as determined by quantitative PCR. (C) Levels of caspase-1 protein in M17-aSyn cells transfected with empty vector before (Vec) and shRNAs against caspase-1 (RNAi).
