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. 2016 Aug 1;113(34):9587–9592. doi: 10.1073/pnas.1610099113

Fig. S2.

Fig. S2.

Purification and in vitro activity of recombinant caspase-1. (A) Elution profile of renatured caspase-1 from a Superdex 75 size-exclusion column. (B) SDS/PAGE of recombinant caspase-1 along the purification procedure, including resolubilization of inclusion bodies with denaturant (DN), refolded rapid dilution (RN), and purification through a Superdex 75 column (FP). The purified fraction consisted predominantly of caspase-1, which is composed of P20 and P10 domains. (C) Standard of fluorescence vs. AMC concentration used for determining the activity of recombinant caspase-1. (D) Activity of assay of our purified recombinant caspase-1 (green diamond, red square, and blue triangle), showing it to be at least as active as commercial positive control (open circle).