Fig. S2.

Expression of human erythropoietin (EPO) decorated with α2,3-sialylated N-glycans. (A) Schematic presentation of the binary vector used to express the full-length human α2,3-sialyltransferase (ST3). ST3 and EPO (16) vectors were coexpressed in ΔXTFT, along with pCe144 (Fig. S1), the CST, and GalT vectors (14). (B) Liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) of EPO tryptic glycopeptide 2 (Asn⁸³, G⁷⁷QALLVNSSQPWEPLQLHVDK⁹⁷). Glycan diagrams were drawn using the Consortium for Functional Glycomics symbols (www.functionalglycomics.org). 35S, cauliflower mosaic virus (CaMV) 35S promoter; g7, Agrobacterium gene 7 terminator; LB, left border; Nos, nopaline synthase promoter or terminator; npII, neomycin phosphotransferase II gene conferring resistance to kanamycin; RB, right border; ST3, human alpha-N-acetyl-neuraminide alpha-2,3-sialyltransferase (NM_006278).