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. 2016 Aug 9;113(34):9623–9628. doi: 10.1073/pnas.1605045113

Fig. 1.

Fig. 1.

Repeated cocaine administration persistently represses PRMT6 expression in the NAc of mice, rats, and addicted humans. (A) Schematic for i.p. injection of saline or cocaine (20 mg/kg) in mice shown in the other panels. (B) NAc mRNA levels of the nine mammalian PRMTs after seven daily i.p. injections of saline (saline) or cocaine (repeated cocaine) or six daily saline treatments and one cocaine injection (acute cocaine). NAc mRNA levels are compared with the NAc of mice treated with seven daily i.p. saline injections. Shown are NAc mRNA levels of Prmt1 [F(2,69) = 4.36, P = 0.016; saline vs. acute: t(41) = 2.42, P < 0.05; saline vs. repeated: t(52) = 2.57, P < 0.01], Prmt2 [F(2,52) = 4.23, P = 0.02; saline vs. repeated: t (36) = 2.91, P < 0.01], Prmt5 [F(2,70) = 4.62, P = 0.013; saline vs. repeated: t(53) = 3.02, P < 0.01], Prmt6 [F(2,53) = 3.72, P = 0.03; saline vs. repeated: t (36) = 2.53, P < 0.01], Prmt8 [F(2,27) = 3.41, P = 0.049, saline vs. repeated: t (17) = 2.61, P < 0.01], and Prmt9 [F(2,27) = 6.31, P = 0.006; saline vs. repeated: t(27) = 3.49, P < 0.01] at 24 h after the last i.p. injection. n = 9–27. #P < 0.05; *P < 0.01. (C) NAc mRNA levels of PRMT6 [t(30) = 2.77, P = 0.014] at 28 d after the last i.p. injection. n = 14–16. #P < 0.05; Bonferroni correction, *P < 0.006. (D and E) Protein levels at 24 h after seven daily i.p. injections of either cocaine (20 mg/kg) (repeated cocaine) or saline (saline). (D) NAc protein levels of PRMT6 [t(26) = 2.44, P = 0.011). n = 9–18. *P < 0.05. (E) NAc protein levels of H3R2me2a [t(37) = 2.12, P = 0.02]. n = 7–30. *P < 0.05. (F) PRMT6 and H3R2me2a protein levels in rat NAc and Prmt6 mRNA levels in human postmortem NAc after cocaine exposure. Shwon are NAc rat protein levels of PRMT6 [t(14) = 2.51, P < 0.05] and H3R2me2a [t(14) = 2.38, P < 0.05] after 10 d of either saline or cocaine self-administration (SA) at 1 mg/kg/infusion for 2 h (FR1;TO30s), followed by 7 d of withdrawal with same animals given a single SA reexposure session with either saline or cocaine at 24 h after the reexposure test. n = 5–11. Also shown is the NAc human mRNA level of Prmt6 [t(25) = 2.16, P < 0.05]. n = 13–14. *P < 0.05. Data are presented as mean ± SEM.